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C. pBlu Transformation Efficiency Problem (Week 11, Day 1)
1. Assuming the following parameters:
a. 20 μL of plasmid solution used with a concentration of 0.15 μg/μL
b. 250 μL of cold CaCl2 used to make the bacteria competent
c. 1 mL Luria Broth used to feed the bacteria
d. 50 μL of bacteria suspension plated
e. 72 blue colonies observed
2. QUESTION #5: What is the transformation efficiency (transformed cells / μg
plasmid DNA) for this experiment?



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