PLEASE ANSWER THIS CORRECTLY
PART II: Simulating Protein Synthesis
Prepare the DNA template cards and a data sheet for the results.
Use a tabletop to complete this exercise. Using a pair of scissors, cut out each of the 14 DNA template cards and the ribosome. The first DNA card should look like the following:
#1
DNA =
T A C T T A C C G A G A T T C T T G T T T A T C
mRNA=
Prepare a sheet of paper for the purpose of recording the results of the laboratory. On a sheet of printer paper, create a numbered list from one to fourteen along the length of the paper to correspond to each DNA template card.
Express the DNA sequence in codons. Then simulate the process of transcription in mRNA.
Select DNA code card #1. The sequence of nucleotide bases is shown in the example above.
Divide the DNA sequence into its triplet codons:
On the 'DNA' side of the card, use a pencil or pen to mark off the letters into groups of three, representing individual DNA base triplets or codons. It is important to understand the DNA code as a series of triplet codons. For example, separate the DNA sequence of card #1 as follows.
FROM DNA: T A C T T A C C G A G A T T C T T G T T T A T C
TO DNA codons: TAC / TTA / CCG / AGA / TTC / TTG / TTT / ATC
Transcribe the DNA language into mRNA language:
Using the space on the 'mRNA' side of the card, convert the DNA code into the corresponding complementary mRNA code, following base pairing rules learned in the lab background. Using a pencil or pen, convert each DNA base into the corresponding mRNA base. Remember that in RNA, the base Uracil is substituted for the DNA base Thymine.
If the step was performed correctly, the complementary card should read:
DNA: TAC / TTA / CCG / AGA / TTC / TTG / TTT / ATC
mRNA: AUG / AAU / GGC / UCU / AAG / AAC / AAA / UAG
Fold over the template card in half, along the line located in between the DNA code and the mRNA code. Refer only to the mRNA code for the steps of Translation (in Step 3, below).
Repeat this same process (in step 2, series a through f) to also converting the DNA sequences into mRNA for template cards numbered 2 through 14.
When you are done all 14 DNA template cards should include the assigned DNA sequence, broken into triplet codons, and the codons of the complementary mRNA sequence.
To navigate through hotspots, hit Tab to select an item, then Enter to expand its contents.DNA Template card #1 is shown. The top half is labeled in typed letters DNA= TACTTACCGAGATTGTTTATC. Next is a line, hand written in pencil which says: TAC/TTA/CCG/AGA/TTG/TTT/ATC. Then there is a line, bisecting the card. Below the line is typed mRNA. Then written in pencil is: AUG/AAU/GGC/UCU/AAC/AAA/UAG.
