In this experiment, a plasmid named pARA-R which contains 1. (.......................):gene of interest, was introduced into bacteria by using 2. (.....................) technique. Previously, this type of bacteria was 3. (.......................) treated that altered the cell wall for DNA to get through into the bacteria. The technique was performed at 4. (.................... °C for .................. seconds) to improve the DNA uptake. Then, the transformed cells were cultured in LB media for 15 minutes to allow the expression of 5. (.......................) antibiotic resistance gene in the plasmid. This antibiotic resistance gene codes for the 6. (....................) enzyme and allowed the thriving plasmid-transformed bacteria to continue growing. The transcription of GOI was controlled by 7. (....................) promoter and araC protein which bound to promoter and blocked the binding of 8. (.......................) to the plasmids. However, the addition of 9. (......................) molecules in the plate could inhibit the action of the araC protein and resulted in the expression of rfp protein by the appearance of 10. (.....................) on the plate.