RFLP Case Study: Art Heist
Cut the following DNA samples using the restriction enzyme
BamHI. To indicate a cut, choose a different font color,
highlighting color, or draw directly on the document to
indicate the location of the cut. Note the size of each fragment
produced underneath each sequence.
Sample Found on Security Guard:
BamHI 5....GGATCC.....3
3.....CCTAG G.....5
TTCGGATCCTGATCAGGATCTAAGCTTGCTAGTGATGTAAGGATCCTCTGAA
AAGCCTAGGACTAGTCCTAGATTCGAACGATCACTACATTCCTAGGAGACTT
Suspect 1:
TTCGGATCCTGATCAGGATCCAAGCTTGCTAGTGATGTAAGGATCCTCTGAA
AAGCCTAGGACTAGTCCTAGGTTCGAACGATCACTACATTCCTAGGAGACTT
Suspect 2:
TTCGGATCCTGATCAGGATCTAAGCTGGCTAGTGTTGTAAGGATCCTCTGAA
AAGCCTAGGACTAGTCCTAGATTCGACCGATCACAACATTCCTAGGAGACTT
Draw the bands produced from restriction digest by BamHI on the gel in the
appropriate location next to the DNA Ladder.
Lane 1: DNA Ladder
90 nt
Lane 2: Sample from Victim
75 nt
Lane 3: Suspect 1
50 nt
Lane 3 Suspect 2
30 nt
20 nt
15 nt
10 nt
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