LO60 Explain how PCR is applied in different technologies If you get a sample from a patient that wants to know if they have Covid-19 and you have a real-time PCR machine, what would you need to do to the sample before it goes in the machine considering SARS-CoV2 is a virus with an RNA-based genome.
a. Amplify the sample using CRISPR
b. Denature the RNA strand by increasing the temperature
c. Use a reverse transcriptase to get a DNA strand
d. Create a library of possible target proteins
e. Find the complementary RNA sequence of the gene of interest