Krebs cycle is equilibrium at 25°C with coupled fatty acid biosynthesis. Calculate the free energy of the fatty acid biosynthesis (in kcal/mol), accounting for the concentrations of Oxaloacetate – 0.005 mM; Acetyl-CoA – 0.03 mM; Citrate – 0.001mM and Isocitrate – 0.06 mM.