Answered

You want to use pGLO to produce red fluorescent protein (RFP) instead of green fluorescent protein (GFP). To do so, you cut out the GFP gene from pGLO with two restriction enzymes, and replace it with the coding sequence for RFP. Which two restriction enzymes will you choose to cut the RFP gene (out of a different plasmid) prior to ligating it into the pGLO plasmid? Group of answer choices EcoRI NheI NdeI EcoRV PstI