What was an effect of the Protestant Reformation on English colonization of the New World in the 1500s? a.) The colony of Roanoke was established to spread the Protestant faith in the New World. b.) England united with other Protestant nations to increase their explorations in the New World. c.) Religious turmoil in Europe delayed England’s attempts to build an empire in the Americas. d.) Queen Elizabeth vigorously supported New World exploration as a way to spread Protestantism.