Using clone 2, you induce expression to drive expression of CTC1 . To ensure you’re maximizing the expression, you do a time course and isolate mRNA. You do cDNA synthesis and quantitative RT - PCR . Looking at your CT values across comparing hour 0 and hour 2, how many more copies of CTC 1 are there?

A. Hour 0 = Ct 30
B. hour 1 = Ct 24
C. hour 2 = Ct 20
D. hour 4 = Ct 19