The genome of the bacteriophage M13 was modified to include a CRISPR-Cas 9 operon with the following features: The operon includes a gene coding for protein Cas 9 and a gene coding a sgRNA. The CRISPR-Ca9 operon is under the control of a bacterial constitutive promoter. The sgRNA gene includes a spacer flanked by two repeats. The spacer's sequence matches that of an antibiotic resistance gene present in an antibiotic- resistant form of bacteria. Bacteria containing the antibiotic gene do not contain any sequence matching the phage M13 genome in their CRISPR array. Note: M13 is a lysogenic phage that does not induce bacterial lysis upon infection.

1) You need to predict the outcome of three different experiments. In each experiment, the same M13-modified phage (M13_MDR) infects a different version of the antibiotic-resistant bacteria.
a) In the first experiment, the E. coli genome contained a single copy of an antibiotic resistance gene. Identify the correct statements in the following list and briefly explain why statements are correct or incorrect.

A. Upon infection, the operon contained in the M13_MDR genome is expressed,and a programmed CRISPR surveillance complex targets the bacterial genome.
B. Immediately after infection, the bacterial CRISPR array produces a programmed CRISPR targeting the M13_MDR genome.
C. The sequence of the antibiotic resistance gene targeted by the M13-DMR encoded CRISPR-Cas9 complex starts with a PAM sequence.
D. The synthes is of the programmed CRISPR surveillance complex is likely to lead to the death of the infected bacteria.