A capillary zone electrophoresis was used for the electrophoretic separation of chlorophenol red and indigo carmine dyes. A solution containing known concentrations of chlorophenol red and indigo carmine dyes was prepared in a 100-mL beaker. After the addition of other necessary reagents, the capillary (50cm) was deepened into the sample solution. A potential of 20 kV was applied, which resulted in the migration of the analytes towards the detector side, which was placed 40 cm from the injection end of the capillary. The migration rate of chlorophenol red was 0.65mms.
(a) How long will it take for the chlorophenol analyte to reach the detector?
(b) If the Indigo carmine dye molecule reach the detector after 12 min, What will be its flow rate?
(c) Comment on the separation efficiency of the two compounds.