During eukaryotic transcription, an RNA molecule is formed that is D. complementary to part of one strand of DNA. RNA is a single-stranded sequence of nucleotides. It is synthesized from a DNA sequence in the process of transcription. In transcription, only one strand of DNA is used as a template for the synthesis of RNA molecule using the rules of complementarity (adenine binds to thymine, cytosine binds to guanine).