Answer :
the one that negatively influenced colonists' opinions of the American indians in Virginia during the early 1600s was :
c. the destruction of soil due to sugar crops
Back then, we have very little knowledge regarding Soil's effectivity. When Sugar canes were heavily populated in a small area, it will degrade the populated soil and caused erotion. ( but of course , the Indians were not doing it with that bad intention)
The answer would be the attack on Jamestown colonists in 1622, here's proof..