Which enzymes are needed to produce recombinant plasmids to be used in gene transfer?
A. DNA polymerase and DNA ligase
B. DNA polymerase and restriction enzyme (endonuclease)
C. Transcriptase and RNA polymerase
D. Restriction enzyme (endonuclease) and DNA ligase
The answer is D. For producing recombinant, first you need to cut the insert part and vector part to get the same end by using restriction enzyme. Then using DNA ligase to bind these two parts to get a new plasmids.
