ANSWER:
The best resin(s) to be used for separation depends on protein size. Sephadex G-10, G-15, and G-25 are suitable for proteins with lower molecular weight
The best resin(s) to be used for separation of the three proteins in Unknown mixture 10 depends on the molecular weight of the proteins.
In gel filtration chromatography, resins
with different pore sizes are used to separate molecules based on size. The Sephadex G series resins are commonly used for this purpose. In this case, Sephadex G-10, G-15, and G-25 should be selected as they have smaller pore sizes and can effectively separate proteins with lower molecular weight.
Here is a breakdown of the Sephadex G series resins and their corresponding pore sizes:
• Sephadex G-10: Pore size range of 1-10 kDa
• Sephadex G-15: Pore size range of 5-15 kDa
• Sephadex G-25: Pore size range of 10-25 kDa
