The Michaelis-Menten rate equation for an enzyme subject to competitive inhibition is
V0=Vmax[S]/αKm+[S]
Beginning with the same Michaelis-Menten starting point,
V0=k2[ES]
a new definition of total enzyme as
[Et]=[E]+[ES]+[EI]
and the definitions of α and KI provided, derive the rate equation for competitive inhibition.
Use the derivation of the Michaelis-Menten equation as a guide, developing a term for [ES].
In the previous steps, you derived the equations
[Et]=[E]+[ES]+[E][I]/KI
[Et]=[ES]+[E](1+[I])/KI
[Et]=[ES]+[E]α
[E]=[ES]Km/[S]
Now substitute equation 6 into equation 5. Simplify the resulting equation such that it only has one term on the right side.
[Et]= ______
The substituted version of equation 5 will become equation 7 in the derivation.