To prepare a 50 mL of a 0.5 M solution from a 10 M stock solution in the lab, you would follow these steps:
1. Calculate the amount of stock solution needed using the formula:
(M1)(V1) = (M2)(V2)
Where:
M1 = concentration of stock solution (10 M)
V1 = volume of stock solution needed (unknown)
M2 = final concentration (0.5 M)
V2 = final volume (50 mL)
Plug in the values and solve for V1 to determine the volume of the stock solution required.
2. Use a graduated cylinder or pipette to measure out the volume of the stock solution calculated in the previous step.
3. Transfer the measured stock solution into a suitable container (such as a beaker).
4. Add distilled water slowly to the stock solution while stirring until the final volume reaches 50 mL. Ensure thorough mixing to obtain a homogeneous solution.
5. Check the final concentration of the solution using a pH meter or a spectrophotometer if necessary to confirm it is indeed 0.5 M.
By following these steps, you can accurately prepare a 50 mL of a 0.5 M solution from a 10 M stock solution in the lab.
