Answer:
a
Explanation:
a. using safranin as a primary stain
Using safranin as a primary stain in Gram staining is not likely to cause a gram-positive cell to appear gram-negative. Safranin is typically used as the counterstain in the Gram staining process to colorize gram-negative cells pink or red, while gram-positive cells retain the crystal violet-iodine complex and appear purple or blue. Counterstaining with crystal violet instead of safranin, exposing the cells to ethanol for too long, or using older bacterial cultures can potentially cause a gram-positive cell to appear gram-negative after Gram staining.
