The disadvantage of tissue culture is that a lab facility with aseptic conditions is required. This means that the environment needs to be completely free from contaminants like bacteria, fungi, or other microorganisms that could negatively affect the growth of the cultured tissues. Maintaining these sterile conditions can be challenging and costly.
Additionally, tissue culture can lead to the production of plants with identical traits, which may limit genetic diversity. This lack of genetic variation can make the plants more susceptible to diseases and environmental changes since they all share the same weaknesses.
Moreover, while tissue culture is efficient at producing exact copies of plants, slight variations from the parent do not usually occur. This lack of genetic variation can be a disadvantage in breeding programs where genetic diversity is essential for developing new and improved plant varieties that are better adapted to changing conditions or possess desirable traits.
