Final answer:
Helicase unwinds DNA strands before replication occurs with the help of single-strand binding proteins and topoisomerase.
Explanation:
Helicase is the protein responsible for unwinding DNA strands before replication. It functions at the origin of the replication fork by using ATP for energy. Single-strand binding proteins then bind to the separated strands, preventing them from rejoining, while topoisomerase helps relieve tension in the DNA during replication.
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