Helicase unwinds DNA strands before replication occurs with the help of single-strand binding proteins and topoisomerase.
Helicase is the protein responsible for unwinding DNA strands before replication. It functions at the origin of the replication fork by using ATP for energy. Single-strand binding proteins then bind to the separated strands, preventing them from rejoining, while topoisomerase helps relieve tension in the DNA during replication.
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