DNA Profiling
NAME:
1. What is a restriction enzyme and what is its role in this process?
2. Describe the use of gel electrophoresis in DNA profiling including the role of the
electric current and fragment sizes.
3. Which way does DNA travel and why? What charge does DNA have?
4. Analyze the DNA profile to determine the paternity of the baby.
5. Identify other possible uses of DNA profiling.
Standard

Aratriction enzyme; enzyme that cuts the
DNA at a specific code.
There are thousands of restriction enzymes.
Each cuts DNA at a different sequence.
Some look for GGCC and cut in between the G
and C.
Every time GGCC is found in the DNA It is cut
by the restriction enzyme
DNA Code:
⚫TTATGGCCATACGGCCTT
EXAMPLE How
to cut DNA
ATAGTGGCCTATA
ATAGTGG CCTATA
Bagments
USE this restriction enzyme to "cut" (add spaces like example above) the DNA strands, and then com
boxes for each on the grid... See example below and on the grid.
Standard
CCAAGACATTATGCAGATGGCCAATAGACATTACGGCCATACCAGAGGCCCAACATGGCCAAACACACCCATCA
Mom
CCTAGACGGCCAGGCACAAGCCAGGCCATGGCCACATCAGTTAGACCGAGGCCGAATCAGGCCTTATTGCAGO
Dad
CCGAGGCCAGGGTTACCGGTATAGGCCAATTGGCCGGCATGGGCCGATACAGCCGATTGGCCATATAGGGGG
Gardener
CCGGTATTCATTACCAGGCCAAGGATACGGCAAGCAGGCCTTCGGCCAATTGGCCTTAGCACGCCGGCCAATO
Baby
CCACATCAGGTAGACCGAGGCCAATTGGCCAACCGACGGCTTAAGGCCCGACAGGCCAAAGACGGCCATATA



Answer :

Final answer:

Restriction enzymes cut DNA at specific sequences while gel electrophoresis separates DNA fragments based on size, aiding in DNA profiling and identifying paternity.


Explanation:

Restriction enzymes are proteins that cut DNA at specific sequences, acting like molecular scissors. For example, the enzyme EcoR1 cuts at the sequence GAATTC. These enzymes are crucial in DNA profiling as they help in cleaving DNA molecules for analysis.

Gel electrophoresis is used in DNA profiling to separate DNA fragments based on size. The electric current aids in pushing the DNA fragments through the gel, with smaller fragments traveling further. By comparing fragment sizes on the gel, different DNA profiles can be distinguished.

DNA travels from the negative to the positive electrode in gel electrophoresis due to its negative charge. Since DNA is negatively charged, it moves towards the positively charged electrode. Analyzing DNA profiles helps determine paternity by comparing the banding patterns between individuals. Other uses of DNA profiling include solving crimes, identifying genetic diseases, and determining relationships among individuals.


Learn more about DNA profiling here:

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