Which step is missing in the following aseptic procedure of inoculating a slant
culture from a pure culture of bacteria (inoculum): 1) flame loop; 2) pull inoculum
tube cap; 3) flame tube top; 4) obtain inoculum on loop and withdraw from tube; 5)
flame inoculum tube; 6) cap tube; 7) pull cap off sterile medium tube; 8) flame top of
tube; 9) inoculate medium and withdraw the loop; 10) flame tube top; 11) cap tube,
and 12) set loop down.
Sterilizing loop before resting it down on lab bench
Flaming top of inoculum tube following obtaining inoculum
Putting cap back on freshly inoculated medium
Initial flaming of inoculum tube top
All steps are present



Answer :

Final answer:

The missing step in the aseptic inoculation procedure is the initial flaming of the inoculum tube top.


Explanation:

The missing step in the aseptic procedure of inoculating a slant culture from a pure culture of bacteria is initial flaming of the inoculum tube top. This step is crucial to ensure the sterilization of the top of the inoculum tube before obtaining the inoculum on the loop.


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