Flaming the mouth of the tube before introducing the specimen is necessary to prevent contamination. The inoculating loop should be cooled by touching the broth, not plunged in, to avoid killing bacteria.
False. Flaming the mouth of the tube containing sterilized nutrient broth is necessary before introducing a specimen to prevent contamination. False. After sterilizing the inoculating loop, it should be immediately cooled by touching it to the broth bacterial culture, not plunged in, to avoid killing the bacteria.
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