Final answer:
The correct restriction enzyme to cut the 5' end of the GFP gene is HindIII, generating sticky ends for DNA fragments.
Explanation:
The restriction enzyme you would use to cut the 5' end of the GFP gene is HindIII.
HindIII is a restriction enzyme that recognizes the specific sequence 5'-AAGCTT-3' and cuts between the A and G bases, producing sticky ends.
EcoRI generates sticky or cohesive ends, and BamHI cleaves DNA at a specific 6-base sequence.
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