Answer :

To determine the minimum number of amino acids required for a chain to be considered a polypeptide, let's review the concept of a polypeptide within biological definitions.

A polypeptide is a long, continuous, and unbranched peptide chain comprised of amino acids. Generally, in biological systems, a polypeptide must meet a certain length to be considered functional as a protein or protein fragment, typically consisting of at least a specific number of amino acids.

Given this information:
- The minimum threshold for a chain to be considered a polypeptide is important for its biological functionality.

From the options provided:
○ 100
150
○ 400
○ 300

The correct choice indicating the minimum number of amino acids that need to be arranged in a chain for it to be classified as a polypeptide is 100. Therefore, the answer is:

○ 100

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