Chargaff's team utilized base composition analysis to compare nitrogenous base amounts in DNA samples by determining the relative concentrations of A, T, C, and G based on Chargaff's rules.
Chargaff's team used a technique called base composition analysis to compare the relative amounts of nitrogenous bases in DNA samples. This involved determining the concentrations of the A, T, C, and G bases by hydrolyzing DNA from various species into individual pyrimidines and purines. Based on Chargaff's rules, if a DNA sample has 22% thymine, then adenine would also be 22%. Since A equals T and G equals C, cytosine and guanine would also be 28% each to maintain the balance.
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