Answered

Which one of the following may cause a FALSE-NEGATIVE result with antiglobulin techniques?

Please select the single best answer:

A. Dust or dirt is present in the glass tubes used for antiglobulin testing.
B. Patient or donor blood specimen was collected in silicone gel tubes.
C. Patient or donor blood specimen has a positive DAT.
D. Addition of AHG reagent is delayed for 40 minutes or more after final saline wash.



Answer :

GinnyAnswer

Final answer:

Delay in adding AHG reagent can cause a false-negative result in antiglobulin techniques.


Explanation:

Addition of AHG reagent is delayed for 40 minutes or more after final saline wash may cause a FALSE-NEGATIVE result with antiglobulin techniques. For example, if the AHG reagent is added late, it might not adequately detect the antigen-antibody reactions, leading to a false-negative outcome. This delay can impact the accuracy of the test results and hinder the identification of important antibodies present.


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