26. Syphilis can be most easily diagnosed in a patient by

A. observing the spirochete under a bright-field microscope.
B. preparing an electron microscope preparation.
C. using Gram-stain and a bright-field microscope.
D. placing scrapings under a dark-field microscope.

27. Staining techniques are useful in microbiology because

A. most microscopes are of poor quality.
B. the cytoplasm of microorganisms is transparent.
C. stains deflect electrons propagated toward the slide.
D. stains make the condenser and diaphragm of the microscope unnecessary.

Syphilis can be diagnosed by observing Treponema pallidum under a darkfield microscope. Staining techniques enhance visibility for microbiology diagnosis. Darkfield microscopy is useful for live specimen observation, while brightfield microscopy with staining aids in visualizing microorganism features.

Explanation:

Syphilis diagnosis: Syphilis can be diagnosed in a patient by observing the spirochete under a darkfield microscope. The causative agent, Treponema pallidum, is best viewed using this method without the need for staining.

Staining techniques: Staining techniques are useful in microbiology because they enhance contrast and visibility. The preferred methods for diagnosing early syphilis include darkfield or brightfield microscopy with silver stain to detect T. pallidum.

Brightfield vs. Darkfield microscopy: Darkfield microscopy is ideal for observing live specimens like thin spirochetes, such as T. pallidum, without staining. Conversely, brightfield microscopy with staining helps in visualizing certain characteristics of microorganisms.

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