Helicase is the enzyme likely affected when DNA strands fail to separate for replication.
The enzyme most likely affected when the DNA strands do not separate for replication is helicase.
Helicase is responsible for unwinding the DNA double helix, creating the replication fork where DNA synthesis occurs.
If helicase is dysfunctional, the DNA strands cannot separate, hindering the initiation of the replication process.
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