Final answer:
Different restriction enzymes should be used when making recombinant DNA.
Explanation:
False: The same restriction enzymes should never be used to cleave the DNA from both sources when making recombinant DNA because it can lead to undesirable outcomes. Using the same enzyme can result in the DNA fragments having the same ends, making it difficult to create properly recombined DNA.
It is recommended to use different restriction enzymes for each DNA source to produce complementary ends that can easily bind together when creating recombinant DNA. This approach ensures successful and efficient recombination of DNA from different sources.
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