Mitochondria's separate DNA and characteristics suggest they were once independent organisms before forming symbiotic relationships within cells.
Mitochondria have their own DNA, separate from the nucleus, indicating that they were once free-living prokaryotes that formed a symbiotic relationship with ancient eukaryotic cells.
This is in line with the endosymbiotic theory, where mitochondria were engulfed by larger cells and provided energy in exchange for protection.
Features such as their own ribosomes and DNA, ability to reproduce independently, and resemblance to prokaryotic division all support the theory that mitochondria were once free-living organisms.
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