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Lab Related

1. Why is it essential that you avoid mixing/agitating thioglycollate broth at all points in this lab?

A. Mixing can disrupt the oxygen gradient in the media.
B. Mixing can change the nutrient gradient in the broth.
C. Mixing increases the risk of contaminating the sample.
D. Mixing can disperse the bacteria into areas where they did not actually grow.
E. Mixing can alter the aerotolerance of the samples inoculated into the media.



Answer :

GinnyAnswer

Final answer:

Avoid mixing thioglycollate broth to preserve different oxygen environments needed for bacterial growth.


Explanation:

It is essential to avoid mixing/agitating thioglycollate broth in the lab to maintain the oxygen gradient within the media. Thioglycollate medium is designed to test the aerotolerance of bacteria by creating different oxygen environments in the tube. Mixing the broth can disrupt this gradient, affecting the growth of bacteria with varying oxygen requirements.


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