Select the true statement(s) about thioglycollate broth (select all that apply).

A. The dye resazurin removes oxygen from the media.
B. This media is useful for growing fastidious microbes.
C. This media can grow organisms that may not tolerate atmospheric oxygen levels.
D. When fresh, this media is normally pink throughout.
E. Thioglycollate broth is used to detect the presence of pathogens in clinical blood samples.



Answer :

Final answer:

Thioglycollate broth is a medium for testing bacterial aerotolerance, supporting the growth of organisms intolerant to oxygen and helpful for anaerobic bacteria. Resazurin serves as an indicator for oxygen presence.


Explanation:

Thioglycollate broth is a medium used to test the aerotolerance of bacteria, with a reducing agent that removes oxygen from the media. It allows the growth of organisms that may not tolerate atmospheric oxygen levels and is particularly useful for growing anaerobes. The indicator resazurin is pink in the presence of oxygen, aiding in the differentiation of aerobic and anaerobic zones.


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