Final answer:
DNA mutations during replication can impact an organism's phenotype, with some mutations being harmful, especially if they affect key genetic sequences.
Explanation:
Mutations can occur during DNA replication due to errors in the polymerization of the second strand, leading to changes in an organism's phenotype if they affect protein-coding sequences. These errors are called mutations, with typical error rates of 1 error in every 10–100 million bases. While most mutations are not harmful, some can be deleterious, such as those impacting cell cycle regulation and potentially causing cancer.
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