Final answer:
Ligase is not involved in the initiation of DNA replication, unlike primase, ligase, and topoisomerase.
Explanation:
Ligase is not involved in the initiation of replication in DNA. It is responsible for joining the Okazaki fragments together during replication. Topoisomerase is an essential enzyme that helps in relieving the strain generated during unwinding of the DNA double helix. Primase is crucial for synthesizing RNA primers that DNA polymerase can extend during replication.
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