Louis Pasteur's experiment showed that microbial growth in the broken necked flask was due to the introduction of microorganisms from the air after the neck was broken.
During Louis Pasteur's experiments, the broth in the broken necked flask showed growth because microbes in the air then had access to the broth. The broth was not sterilized by the heat. When the neck of the flask was broken, it allowed microorganisms from the air to contaminate the previously sterile broth, leading to microbial growth.
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