Answered

Indicate what could happen if whole blood overlaid on Histopaque is centrifuged as follows:

A. Higher speed was used.
B. Higher temperature than requested was used when centrifuging.
C. Lower speed was used.
D. Blood wasn't overlaid but poured into Ficoll Histopaque.
E. Water was used instead of Ficoll Histopaque.



Answer :

GinnyAnswer

Final answer:

Explains the outcomes of centrifuging whole blood on histopaque under different conditions.


Explanation:

When whole blood is overlayed on histopaque and centrifuged with variations in speed, temperature, or using different substances, different outcomes can occur:

  1. If higher speed is used: Components may separate more efficiently based on density.
  2. If high temperature is used: It can affect the integrity of the blood components during centrifugation.
  3. If lower speed is used: Separation may be less effective and results may be inaccurate.
  4. If blood wasn't overlayed but poured into ficoll histopaque: Proper separation may not occur.
  5. If water was used instead of ficoll histopaque: There would be no density gradient for separation.

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