Final answer:
Explains the outcomes of centrifuging whole blood on histopaque under different conditions.
Explanation:
When whole blood is overlayed on histopaque and centrifuged with variations in speed, temperature, or using different substances, different outcomes can occur:
- If higher speed is used: Components may separate more efficiently based on density.
- If high temperature is used: It can affect the integrity of the blood components during centrifugation.
- If lower speed is used: Separation may be less effective and results may be inaccurate.
- If blood wasn't overlayed but poured into ficoll histopaque: Proper separation may not occur.
- If water was used instead of ficoll histopaque: There would be no density gradient for separation.
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