Spectrophotometry involves using light-matter interaction to determine substance concentrations. Beer's Law explains the relationship between color and concentration. Spectrophotometers measure light absorption to quantify substance concentrations.
Spectrophotometry is a technique used to determine the concentration of substances in solution based on the interaction of light and matter. In spectrophotometric analysis, an unknown concentration can be determined by measuring the absorbance of a sample at specific wavelengths and comparing it to a standard curve generated from known concentrations of the substance.
The relationship between color and concentration in spectrophotometry is described by Beer's Law, which states that the absorbance of a substance is directly proportional to its concentration. This means that as the concentration of a substance increases, the absorbance of light by that substance also increases.
Spectrophotometers function by emitting a range of electromagnetic waves, with the sample absorbing some of these waves. By measuring the intensity of light that passes through the sample, the concentration of the absorbing species can be quantitatively determined using the Beer-Lambert Law.
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