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Proposed Work

Aim 1: Determine a mechanism for acetate-activated xylose metabolism

1.1 Test if acetate affects xylose metabolism at lower nitrogen content
1.2 Test if increasing acetate metabolism affects xylose metabolism

Aim 2: Enhance the yield on xylose without acetate

2.1 Test if phosphoketolase (XFPK) deletion impairs biomass yield on xylose
2.2 Test if XFPK and PTA overexpression improves biomass yield on xylose
2.3 Test if the best lipid-producing strain (ADS) endowed with XFPK improves lipid yield on xylose

### Notes:
- XYLY
- 3029
- xylase
- KDW
- xylitol
- ACC-20A
- AcO
- Ac-CoA
- ALS
- PTL
- Ac-P
- GAJP
- xylulose
- XuSP
- ADH
- Ru5P
- PATION
- phosphere
- abitol
- nbulose



Answer :

GinnyAnswer

Final answer:

Explore mechanisms for acetate-activated xylose metabolism and enhancing xylose yield without acetate in biological processes.


Explanation:

Aim 1: Mechanism for acetate-activated xylose metabolism

  1. Testing acetate's effect on xylose metabolism at lower nitrogen content.
  2. Investigating how increased acetate metabolism impacts xylose metabolism.

Aim 2: Enhancing xylose yield without acetate

  1. Examining the impact of phosphoketolase (XFPK) deletion on biomass yield.
  2. Testing the effect of XFPK and PTA overexpression on biomass yield.
  3. Assessing the lipid yield on xylose with the ADS strain endowed with XFPK.

The process of turning xylose into hydrogen with high yield using enzymes, including polyphosphate xylulokinase (XK), was developed at low temperature.

Activation of carboxyl end of acetate is crucial for providing acetyl-CoA needed for various metabolic pathways.

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