DNA ligase is the enzyme that repairs nicks or breaks along the DNA backbone by catalyzing the formation of a phosphodiester bond between nucleotides.
DNA ligase is the enzyme that repairs nicks or breaks along the DNA backbone by catalyzing the formation of a phosphodiester bond between nucleotides. It plays a crucial role in joining DNA strands together and repairing single-strand breaks in DNA.
For example, DNA ligase can seal breaks in the DNA backbone that occur during DNA replication, DNA damage, or the DNA repair process. It uses the complementary DNA strand as a template to repair single-strand breaks by creating phosphodiester bonds.
Unlike DNA ligase, restriction enzymes hydrolyze phosphodiester bonds in DNA strands, producing different types of ends that are crucial for DNA manipulation and cloning.
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