What do you want to accomplish when making smears of bacteria that will be stained and
viewed under the microscope?
(Select all that apply)
Prevent cell shrinkage.
Prepare a thick smear to increase the chances of seeing something under the microscope.
Adhere cells to a glass slide so they do not wash away when stained.
Prepare a thin smear for better visualization.
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Bacterial smear preparation involves fixation to prevent cells from washing away during staining and aid in visualization under the microscope. Different methods are used depending on whether the bacteria are from liquid or solid culture. The process includes spreading a thin film of culture on a slide.

Explanation:

Importance of Fixation in Bacterial Smear Preparation:

Fixation is crucial to adhere bacterial cells to the slide, preventing them from washing away during staining. It also kills the bacteria, aiding in visualization under the microscope.

Differences in Smear Preparation:

From liquid culture: A loopful of culture spread on the slide.
From solid culture: A drop of water on slide, then a small amount of growth obtained and spread.

Preparation of Bacterial Smear:

Smear preparation involves spreading a thin film of culture on a slide to facilitate staining.

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